we test the hypothesis that inhibition of GSK will stimulate glycogen synthesis

HPLC analysis indicated that the DG extract contained the fol lowing danshensu, marker ingredients, salvianolic p T, protocatechuic aldehyde, puerarin, daidzein 8C apiosyl glucoside, daidzin and daidzein. 15 gkg. All experimental methods were approved by the Research Practice Committee at the HKUST. Induction of acute Fingolimod cost myocardial injury Animals were randomly assigned to different groups of six animals in each for the induction of myocardial injury with or without post treatment with the DG extract. Animals received an intraperitoneal injec tion of ISO at single-dose of 200 mgkg for the induction myocardial injury. Pre liminary studies indicated that the ISO administration increased plasmenzyme actions within six hours in the rats. Get a grip on animals received the car only. Blood samples were obtained from phenobarbital anesthetized rats at increasing time intervals post ISO administration. These subjects were then sacrificed by removal. Myocardial ventricular tissue samples were obtained for the preparation of cytosolic and mitochondrial fractions for biochemical analyses. Basal values of Organism plasmenzyme actions and myocardial mitochondrial parameters were obtained from animals sacrificed immediately after the injection of saline. DG post treatment protocol Animals were intragastrically administered together with the DG extract at dose of 4 gkg just after intraperito neal procedure of ISO in the rat model of ISO induced acute myocardial injury. Initial reports indicated that oral administration of the DG extract at 2 gkg did not produce any detectable changes in plasmenzyme actions four hours after intraperitoneal injection of ISO in rats. Inhibitors of PKC and mKATP PKC translocation inhibitor and 5 hydroxydecanoate, which are inhibi tors of PKC and mKATP respectively, were dissolved in DMSO at focus of 400 ugmL. Rats were injected together with the chemical at 400 ug per kg of body weight for one hour ahead of the intra-gastric administration UNC0638 clinical trial of DG extract or vehicle. Control animals received 1. Six months DMSO in saline. Preparation of plasmsamples and myocardial mitochondrialcytosolic fractions Blood was drawn from phenobarbital anesthetized rats by cardiac puncture in to syringe rinsed with 5% Na2EDTas anti-coagulant. As plasmsamples the superntants were obtained. Myocardial ventricular tissue samples were rinsed with ice cold isotonic buffer. Tissue homogenates were prepared by homogenizing 0.